Specimen Preparation Adjust pH to 1 by adding 6M HCl (approximately 10 mL HCl/24-hour specimen based on normal adult output of 1000-2000 mL/24 hours. Pediatric specimens will require less than 10 mL to reach the correct pH). Record total volume and collection time interval on transport tube and test request form. Transfer 4 mL aliquot of urine from a well-mixed 24-hour collection
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