Sample collection and handling: 1- samples have to be drawn by vaccutainer to the line 2- Order of the blood draw: Nill tube first then TB1 then TB2 and finally Mitogen tube 3- Tubes have to be mixed thouroughly (by inversion 10 times) immediately after blood draw, and incubated straight away at 37 C 4- After incubation is complete, samples should be centrifuged at 2000 - 3000 rpm for 15 min 5- Transfer the supernatenet to another test tubes with a correct labeling 6- Ship samples along with ice packs to maintain the temprature.
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