Samples should be drawn immediately during the attack of fever, otherwise parasites may not be found. Specimen Preparation Transport 5 mL whole blood (Min: 1 mL). Extended exposure to EDTA anticoagulants can result in altered parasite morphology. For best results, send five (5) thin blood smears (unstained, unfixed) AND five (5) thick smears (unstained, unfixed) in addition to whole blood. Thin and thick blood smears should be prepared immediately or within 1 hour after collection. Thin smears are made as per routine hematology differentials. Thick smears are made by dropping 10 to 20 µL of blood onto a slide and spreading it into a dime-sized area with a glass, wooden, or plastic applicator.
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