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The coagulation factors Va and VIIIa are regulated through the Protein C / Protein S pathway. Thrombin bound to thrombomodulin becomes a potent activator of Protein C. In combination with its cofactor, Protein S, activated Protein C (APC) inactivates factor Va and VIIa by specific cleavages, thus, slowing down the coagulation cascade. Factor Va resistance to protein C (Factor V Leiden) is a thrombophilic disorder that is inherited in an autosomal dominant manner. It is now recognized as the single most common cause of hereditary thrombophilia. See Activated Protein C Resistnace (Factor V Leiden), R506Q Mutation, and Protein C, Plasma.
Avoid haemolysed samples. Separate plasma and freeze immediately after collection.Patient should stop anticoagulant therapy before testing (consult treating physician).